US 5,059,654 A · Affinity matrices of modified polysaccharide supports

Abstract

The invention is directed to a modified polysaccharide material which comprises: (1) polysaccharide covalently bonded to a synthetic polymer; (2) the synthetic polymer being made from (a) a polymerizable compound which is capable of being covalently coupled directly or indirectly to said polysaccharide, and (b) one or more polymerizable compounds containing (i) a chemical group capable of causing the covalent coupling of the compound (b) to an affinity ligand or a biologically active molecule or (ii) a hydrophobic compound. The invention is also directed to devices for the chromatographic separation of at least two components of a mixture comprising the modified polysaccharide material of the invention, wherein the device is configured for radial or tangential flow.

Claims28

Dependent claims

A modified polysaccharide material which comprises:

(1) a water-insoluble polysaccharide covalently bonded to a synthetic polymer;
(2) said synthetic polymer made from
(a) a polymerizable compound which has an epoxy group capable of direct covalent coupling to said polysaccharide;
and
(b) one or more polymerizable compounds containing
(i) a chemical group capable of causing the covalent coupling of said synthetic polymer to an affinity ligand or a biologically active molecule, or
(ii) a hydrophobic chemical group.

4 more elements

A chromatography device for effecting chromatographic separation of at least two components of a sample, comprising:

(1) a cylindrical core,
(2) at least a first and second stationary phases wound around said cylindrical core, wherein
(a) a first phase comprises a modified polysaccharide material which comprises:
(i) a water-insoluble polysaccharide covalently bonded to a synthetic polymer;
(ii) said synthetic polymer made from
(1) a polymerizable compound which has an epoxy group capable of direct covalent coupling to said polysaccharide;
and
(2) one or more polymerizable compounds containing
(a) a chemical group capable of causing the covalent coupling of said synthetic polymer to an affinity ligand or a biologically active molecule, or
(b) a hydrophobic chemical group,
wherein said first phase is in the form of a sheet;
(b) a second phase comprising a means for supporting, separating, and providing channels tangential to said cylindrical core;
(3) means for distributing the sample to said channels;
(4) means for collecting the sample from said channels;
and
(5) a cylindrical housing.

13 more elements

Dependencies are inferred from the claim text.

Facts

Application

07/311,498Filed 16 Feb 1989 · continuation in part

Priority

14 Feb 1983

Granted

22 Oct 1991

Term ends

Not estimatedThe source records don’t support an estimate.

Classification32 codes

B01J 20/28052
Characterised by their form: several layers of identical or different sorbents stacked in a housing, e.g. in a column
A61K 39/39525
From serum, plasma: purification
B01D 15/3804
Involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or…
B01D 15/3809
Affinity chromatography: of the antigen-antibody type, e.g. protein A, G or L chromatography

Family

Serial466,114Parent of this continuation-in-part · filed 14 Feb 1983

Serial576,448Parent of this continuation-in-part · filed 2 Feb 1984

Serial656,922Parent of this continuation-in-part · filed 2 Oct 1984

Serial13,512Parent of this continuation-in-part · filed 27 Jan 1987

Serial130,186Parent of this continuation-in-part · filed 8 Dec 1987

Serial154,815Parent of this continuation-in-part · filed 11 Feb 1988

US 4,639,513 AParent of this continuation-in-part

US 4,663,163 AParent of this continuation-in-part

US 5,059,654 AThis patent

From the US filing history. Foreign family members aren’t listed.

Affinity matrices of modified polysaccharide supports

Life of this patent

Affinity matrices of modified polysaccharide supports

Life of this patent

Abstract

Claims28