Mutant strains of azotobacter vinelandii used for the hyperproduction of poly-.beta.-hydroxybutyrate during exponential growth
AssigneeThe Governors of the University of Alberta
InventorsWilliam J. Page, Olga Knosp
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Abstract
A mutant strain of Azotobacter vinelandii exhibits hyper-production of poly-.beta.-hydroxybutyrate during its growth.
Claims5
A biologically pure culture of a mutant microorganism of claim 4 having all the identifying characteristics of ATCC 53799, said identifying characteristics of ATCC 53799 including the hyperproduction of pHB in recoverable quantities during the exponential growth of said mutant microorganism.
A biologically pure culture of a mutant microorganism of claim 4 having all the identifying characteristics of ATCC 53799, said culture being capable of hyperproducing pHB in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable nutrients, said hyperproduction of pHB occurring during the exponential growth of said mutant microorganism.
A biologically pure culture of a mutant microorganism of the species Azotobacter vinelandii, said mutant having the identifying characteristics:
- i) produces cells containing extremely abundant pHB granules in culture;
- ii) forms dense white colonies of cells in solid culture;
- iii) after 24 hours of growth in liquid culture, forms a very turbid culture which reaches an O.D..sub.620 of 10 and looks like cow's milk;
- iv) during exponential growth, hyperproduces pHB but does not product substantial amounts of capsules and does not produce substantial amounts of slime.
Dependencies are inferred from the claim text.