Saponin adjuvant
AssigneeCambridge Biotech Corporation
InventorsCharlotte A. Kensil, Dante J. Marciani
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Abstract
Substantially pure saponins are disclosed. The saponins of the present invention are useful as immune adjuvants. Disclosed as well are immune response-provoking compositions comprising an antigen in admixture with the substantially pure saponins.
Claims16
Substantially pure saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by a single predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, when analyzed on reverse phase-HPLC on a Vydac C.sub.4 column having 5 .mu.m particle size, 330 .ANG. pore, 4.6 mm ID.times.25 cm L in a solvent of 40 mM acetic acid in methanol/water (58/42;
- v/v) at a flow rate of 1 ml/mixture, and wherein said saponin has immune adjuvant activity and is less toxic when used as an adjuvant than said Quillaja saponaria extract.
Substantially pure QA-7 saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by one predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, and having a retention time of approximately 9-10 minutes when analyzed on reverse phase HPLC on a Vydac C.sub.4 column having 5 .mu.m particle size, 330 .ANG. pore, 4.6 mm ID.times.25 cm L in a solvent of 40 mM acetic acid in methanol/water (58/42;
- v/v) at a flow rate of 1 ml/minute.
Substantially pure QA-21 saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by one predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, and having a retention time of approximately 51minutes when analyzed on reverse phase-HPLC on a Vydac C.sub.4 column having 5 .mu.m particle size, 330 .ANG. pore, 4.6 mm ID.times.25 cm L in a solvent of 40 mM acetic acid in methanol/water (58/42;
- v/v) at a flow rate of 1 ml/minute.
A substantially pure QA-17 saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by one predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, and having a retention time of approximately 35 minutes on reverse phase-HPLC on a Vydac C.sub.4 column having 5 .mu.m particle size, 330 .ANG. pore, 4.6 mm ID.times.25 cm L in a solvent of 40 mN acetic acid in methanol/water (58/42;
- v/v) at a flow rate of 1 ml/minute.
A substantially pure QA-18 saponin purified from a crude Quillaja saponaria extract wherein said pure saponin is characterized by one predominant peak which comprises 90% or more of the total area of all peaks of a chromatogram, excluding the solvent peak, and having a retention time of approximately 38 minutes on reverse phase-HPLC on a Vydac C.sub.4 column having 5 .mu.m particle size, 330 .ANG. pore, 4.6 mm ID.times.25 cm L in a solvent of 40 mM acetic acid in methanol/water (58/42;
- v/v) at a flow rate of 1 ml/minute.
Dependencies are inferred from the claim text.