Bovine nuclear transplantation
AssigneeGranada Biosciences, Inc.
InventorJoseph M. Massey
Life of this patent
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Abstract
A process to produce viable bovine embryos through nuclear transplantation. The process utilizes nuclear material from 4 cell and later stage developed embryos recovered from inseminated donor cows through nonsurgical or surgical means. Recipient eggs or oocytes are recovered and a portion of the ooplasm removed by micromanipulation. Individually separated cells from the nuclear material donor embryo are inserted into the recipient oocyte. The oocyte fragment and nuclear donor material are electrofused. The embryo is cultured in vitro, transferred for an interim time to a sheep oviduct and then transferred to a cow for gestation.
Claims20
A process to produce viable bovine embryos through nuclear transplantation comprising the following steps:
- (a) collecting recipient oocytes from donor cows;
- (b) collecting embryos greater than 32 cells;
- (c) removing a portion of the ooplasm from the recipient oocyte;
- (d) separating the embryo cells into single donor cells for nuclear transplantation;
- (e) inserting said donor cell into the recipient oocyte;
- (f) electrofusing the oocyte and donor cell to form an embryo;
- and
- (g) culturing said embryo.
A process to produce viable bovine embryos through nuclear transplantation comprising the following steps:
- (a) collecting oocytes from donor cows;
- (b) collecting embryos greater than 32 cells;
- (c) slitting the zona pellucida of at least one oocyte more than 180.degree.;
- (d) removing the polar body and at least 150 of the ooplasm of the oocyte;
- (e) separating the embryo cells into disassociated blastomeres;
- (f) inserting said blastomeres into the perivitelline space in close contact with the remaining ooplasm of the oocyte fragment;
- (g) electro fusing the oocyte fragment and the blastomere to form an embryo;
- and
- (h) culturing said embryo.
Dependencies are inferred from the claim text.