Genetically engineered plasmid and organisms for the production of specialized oils
AssigneeRutgers University
InventorsCharles E. Martin, Robert M. Johnston
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Abstract
A DNA fragment containing a gene encoding a fatty acid desaturase enzyme is isolated. The gene is then employed by introducing it into other cells to alter or enhance the production of oils in such cells, alternatively the gene or nucleic acid sequences of the gene can be employed to isolate other genes encoding for related, but different desaturase enzymes.
Claims20
An expression vector comprising a yeast gene encoding a delta-9 fatty acid desaturase enzyme which functions in a yeast cell to induce or enhance oil production.
A purified DNA comprising the sequence as described in Table 2.
A yeast organism transformed with a DNA comprising the sequence set forth in Table 2.
A method for producing oil in yeast comprising culturing a yeast stain tranformed with an expression vector comprising a gene encoding a delta-9 fatty acid desaturase enzyme in a medium under conditions of time and temperature sufficient to result in oil production by said yeast.
Dependencies are inferred from the claim text.