US 5,057,414 A · Method for the determination of the activity of serine proteases or serine protease inhibitors

Abstract

The invention relates to a method for the determination of the activity of serine proteases and serine protease inhibitors in plasma or other biological fluids. The only methods hitherto available for rapid and accurate determination of the content of serine proteases or serine protease inhibitors have been very time-consuming and methodologically demanding. In the method according to the invention for the determination of the activity of serine proteases or serine protease inhibitors, specific inhibitors are inactivated by addition of at least one oxidizing agent and, where appropriate, at least one detergent. The addition of an omega-aminocarboxylic acid enhances and observed effects when the serine protease is plasmin.

Claims23

Dependent claims

A method for the determination of the activity of serine proteases or serine protease inhibitors in a sample of plasma or other biological fluids comprising the steps of:

(i) adding at least one oxidizing agent to said sample which oxidizes methionine to methionine sulfoxide at a pH of 7.5 to 8.8;
(ii) adding simultaneously o rafter a preincubation period a plasminogen activator and a first substrate for said plasminogen activator, said first substrate being activated by a said plasminogen activator to yield an enzyme which can be detected by means of a second substrate;
and
(iii) bringing said activated first substrate into contact with said second substrate which second substrate can be detected after conversion by said activated first substrate.

1 more element

Dependencies are inferred from the claim text.

Method for the determination of the activity of serine proteases or serine protease inhibitors

Life of this patent

Method for the determination of the activity of serine proteases or serine protease inhibitors

Life of this patent

Abstract

Claims23