Diagnostic and therapeutic antibody conjugates
AssigneeCenter for Molecular Medicine and Immunology
InventorsLisa B. Shih, Frederick J. Primus, Milton D. Goldenberg
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Abstract
The present invention relates to conjugates of diagnostic or therapeutic principles, such as drugs, toxins, chelators, boron compounds and detectable labels, to an antibody, in which the diagnostic or therapeutic principle is first loaded onto a polymer carrier such as an aminodextran or a polypeptide of at least 50 amino acids in length, and this intermediate is in turn site-specifically conjugated to a targeting antibody such as an antitumor antibody. The resultant conjugate substantially retains the immunoreactivity of the antibody, and targets the diagnostic or therapeutic principle to a target tissue or organ where the diagnostic or therapeutic effect is realized.
Claims32
A soluble antibody conjugate, comprising a plurality of drug, toxin, chelator, boron addend or detectable label molecules covalently bound to a polymer carrier having at least one remaining free amine group, to form a loaded carrier, said loaded carrier in turn being covalently bound through said at least one amine group to the carbohydrate portion of an antibody by a reduced Schiff base linkage;
- wherein said conjugate is soluble in human serum.
A method of preparing a soluble antibody conjugate, comprising the steps of:
- (a) reacting a polymer carrier, to which are covalently bound a plurality of drug, toxin, chelator, boron addend or detectable label molecules, said carrier having at least one remaining free amine group, with an antibody having an oxidized carbohydrate portion;
- and
- (b) reductively stabilizing the resultant Schiff base adduct;
- wherein said conjugate is soluble in human serum.
Dependencies are inferred from the claim text.