Chimeric Bacillus thuringiensis crystal protein gene comprising HD-73 and Berliner 1715 toxin genes, transformed and expressed in Pseudomonas fluorescens
AssigneeMycogen Corporation
InventorThomas E. Gilroy
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Abstract
A novel hybrid B.t. toxin gene toxic to lepidopteran insects has been cloned. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These recombinant microbes can be used to control lepidopteran insects in various environments.
Claims28
DNA encoding a B.t. toxin having the amino acid sequence shown in FIG. 2.
A recombinant DNA transfer vector comprising DNA which codes for the amino acid sequence shown in FIG. 2.
A bacterial host transformed to express a B.t. toxin having the amino acid sequence shown in FIG. 2.
An insecticidal composition comprising insecticide containing substantially intact, treated cells having prolonged pesticidal activity when applied to the environment of a target pest, wherein said insecticide is a polypeptide toxic to lepidopteran insects, is intracellular, and is produced as a result of expression of a transformed microbe capable of expressing the B.t. toxin having the amino acid sequence shown in FIG. 2.
Treated, substantially intact unicellular microorganism cells containing an intracellular toxin, which toxin is a result of expression of a Bacillus thuringiensis toxin gene toxic to lepidopteran insects which codes for a polypeptide toxin having the amino acid sequence shown in FIG. 2, wherein said cells are treated under conditions which prolong the insecticidal activity when said cell is applied to the environment of a target insect.
A plasmid selected from the group consisting of pM2,107-1, pM3,123-1 and pM3,130-7.
Dependencies are inferred from the claim text.