Support for high performance liquid chromatography in a magnetically stabilized fluidized bed
AssigneeUniversity of Pennsylvania
InventorsVictor Goetz, David J. Graves
Life of this patent
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Abstract
A method of, and an apparatus for, conducting chromatographic separations of multi-component systems utilizing silica-coated, magnetically susceptible particles in an MSFB is disclosed. A support material comprised of beads having a magnetic core surrounded by a silica derivative porous coating is used.
Claims28
A method of conducting chromatographic separations of multi-component systems comprising contacting a multi-component system contained in a liquid carrier fluid with a solid support contained in a magnetically stabilized, fluidized support bed, wherein the solid support comprises a plurality of generally spherical beads having a generally central magnetic core and a surrounding exterior coating about the core comprising a porous silica or silica derivative, said magnetic core having a coefficient of expansion similar to or less that than the coefficient of expansion of said porous silica or silica derivative.
A method of conducting High Performance Liquid Chromatography comprising contacting a multi-component liquid solution to be separated with a solid support contained in a magnetically stabilized, fluidized support bed, wherein the solid support comprises a plurality of generally spherical beads having a generally central magnetic core and a surrounding exterior coating about the core comprising a porous silica or silica derivative, said magnetic core having a coefficient of expansion similar to or less than the coefficient of expansion of said porous silica or silica derivative.
A method of conducting chromatographic separations of multi-component systems comprising:
- contacting a multi-component system with a solid support wherein the solid support is continuously fed to the top of a magnetically stabilized fluidized bed and continuously removed from the bottom of the bed, the solid support comprising a plurality of generally spherical beads having
- a generally central magnetic core that is 10-250 .mu.m in diameter,
- an intermediate, non-porous coating comprised essentially of silica, gold, or other protective material, and
- a surrounding exterior coating that is less than 10 .mu.m thick comprising a porous silica or silica derivative
- having a binding ligand capable of binding with one or
- more of the components of the multi-component system wherein the magnetic core is comprised of a material having a coefficient of expansion similar to or less than the coefficient of expansion of said porous silica or silica derivative.
Dependencies are inferred from the claim text.