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US 5,053,332 AGranted
Agarose beads, preferably incorporating biological materials
OwnerRichard B. Cook, Richard B. Provonchee +1inventors
InventorsRichard B. Cook, Richard B. Provonchee, Samuel Nochumson
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Abstract
A method for forming agarose beads, which may incorporate biological material at least partially labile at above 40.degree. C., in which an aqueous agarose solution is formed into bead-size portions which are gelled by contacting the portions with a cooled atmosphere, gas, and/or smooth hydrophobic surface, and then collected.
Claims30
A method for forming agarose beads incorporating biological material at least partially labile at above 40.degree. C. comprising:
- A) forming an aqueous solution consisting essentially of an agarose which gels at a temperature of about 40.degree. C. or less;
- B) cooling the agarose solution to 40.degree. C. or less while maintaining it in liquid form;
- C) admixing an aqueous broth containing biological material which is at least partially labile at above 40.degree. C. with the gel solution, to form a liquid agarose/broth mixture;
- D) forming bead-size portions of the liquid agarose/broth mixture;
- E) contacting said portions with a cooled atmosphere, gas, and/or smooth hydrophobic surface and maintaining such contact until said portions gel into formed agarose beads containing biological material;
- and
- F) collecting the formed beads.
4 more elements
A method for forming agarose beads comprising:
- A) forming an aqueous agarose solution;
- D) forming bead-size portions of said agarose solution;
- E) contacting said portions with a cooled atmosphere, gas, and/or smooth hydrophobic surface and maintaining such contact until said portions gel into formed agarose beads;
- and
- F) collecting the beads.
2 more elements
Dependencies are inferred from the claim text.