# US 5,051,185 A · Absorbent of .beta..sub.2 -microglobulin

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,051,185 A
- Document ID: US5051185A
- Status: Granted
- Kind: A
- Application number: 07/512,629
- Filed: 1990-04-19
- Granted: 1991-09-24
- Earliest claimed: 1987-11-06
- Assignees: Asahi Kasei Kogyo Kabushiki Kaisha; Asahi Medical Co., Ltd.
- Inventors: Hiroyuki Watanabe; Tadashi Yokoyama; Naokuni Yamawaki
- Web page: https://patentgrep.com/patent/US5051185A

## CPC

- A61M 1/3679: {by absorption (A61M1/3675 takes precedence)}
- B01J 20/261: {obtained by reactions only involving carbon to carbon unsaturated bonds (macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F)}
- B01J 20/262: {obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation (macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G)}
- B01J 20/264: {derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers}
- B01J 20/28004: {Sorbent size or size distribution, e.g. particle size}
- B01J 20/28016: {Particle form}
- B01J 20/28023: {Fibres or filaments (fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence)}
- B01J 20/28033: {Membrane, sheet, cloth, pad, lamellar or mat}
- B01J 20/28059: {being less than 100 m2/g}
- B01J 20/28078: {Pore diameter}
- B01J 20/3092: {Packing of a container, e.g. packing a cartridge or column (of chromatography columns B01D15/206)}
- B01J 20/321: {consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds}
- B01J 20/3219: {involving a particular spacer or linking group, e.g. for attaching an active group}
- B01J 20/3255: {comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures}
- B01J 20/3274: {Proteins, nucleic acids, polysaccharides, antibodies or antigens}
- B01J 20/3289: {Coatings involving more than one layer of same or different nature}
- A61M 2202/0421: Beta-2-microglobulin
- B01J 2220/58: Use in a single column
- G03C 2001/0471: {Isoelectric point of gelatine}
- Y10S 210/905: Protein
- Y10S 530/813: Carrier is a saccharide
- Y10S 530/815: Carrier is a synthetic polymer
- A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)}
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- G03C: PHOTOSENSITIVE MATERIALS FOR PHOTOGRAPHIC PURPOSES (for photomechanical purposes G03F); PHOTOGRAPHIC PROCESSES, e.g. CINE, X-RAY, COLOUR, STEREO-PHOTOGRAPHIC PROCESSES; AUXILIARY PROCESSES IN PHOTOGRAPHY (photographic processes characterised by the use or manipulation of apparatus classifiable per se in subclass G03B, see G03B; photomechanical production of textured or patterned surfaces G03F; electrophotography, magnetography G03G)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

An adsorbent for .beta..sub.2 -microglobulin is disclosed, which comprises a water-insoluble carrier having supported thereon, as a ligand, at least one electrolyte selected from the group consisting of a polyamino acid, a polysaccharide, a synthetic high polymer, collagen having an isoelectric point of 9.5 or more, and gelatin having an isoelectric point of 6.5 or more, wherein said electrolyte has a molecular weight not less than 1,000 and an X value of more than 2.0, wherein X is a relationship regarding the skeleton structure of said electrolyte and is the sum of A and B, wherein A represents (the number of carbon atoms of the skeleton structure)-(the number of hydrophilic groups)/(the number of hydrophilic groups); and B represents .vertline.(the number of cationic groups)-(the number of anionic groups).vertline./(the number of hydrophilic groups). The adsorbent exhibits adsorptivity for .beta..sub.2 -microglobulin at high efficiency and high selectivity.

## Claims

### Claim 1

An adsorbent for .beta..sub.2 -microglobulin which comprises a water-insoluble carrier having supported thereon, as a ligand, at least one electrolyte selected from the group consisting of a polyamino acid, a polysaccharide, a synthetic high polymer, collagen having an isoelectric point of 9.5 or more, and gelatin having an isoelectric point of 6.5 or more, wherein said electrolyte contains at least one group exhibiting a positive or negative charge in a neutral electrolytic solution and has a molecular weight of not less than 1,000 and an X value or more than 2.0, wherein X is a relationship regarding the skeleton structure of said electrolyte and is the sum of A and B, wherein A represents (the number of carbon atoms of the skeleton structure)-(the number of hydrophilic groups)/(the number of hydrophilic groups); and B represents.vertline.(the number of cationic groups)-(the number of anionic groups).vertline./(the number of hydrophilic groups).

### Claim 2 (depends on 1)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 1, wherein said ligand is at least one electrolyte selected from the group consisting of a polyamino acid, a polysaccharide and a synthetic high polymer.

### Claim 3 (depends on 2)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 2, wherein said ligand has a molecular weight of from 1,000 to 1,000,000.

### Claim 4 (depends on 2)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 2, wherein said ligand has an X value of from greater than 2 but not more than 15.

### Claim 5 (depends on 2)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 2, wherein said ligand is a polyamino acid electrolyte.

### Claim 6 (depends on 5)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 5 wherein said polyamino acid electrolyte is poly(glutamic acid-alanine) or poly-(glutamic acid-alanine-tyrosine).

### Claim 7 (depends on 2)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 2, wherein said ligand is a polysaccharide electrolyte.

### Claim 8 (depends on 7)

The absorbent for .beta..sub.2 -microglobulin as claimed in claim 7, wherein said polysaccharide electrolyte is diethylaminoethyl-dextran.

### Claim 9 (depends on 1)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 1, wherein said ligand is a synthetic high polymer electrolyte.

### Claim 10 (depends on 9)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 9, wherein said synthetic high polymer electrolyte is sodium sulfonated polystyrene or styrene-maleic acid copolymer.

### Claim 11 (depends on 1)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 1, wherein said water-insoluble carrier has a double-layered structure composed of a water-insoluble material having a contact angle with air bubble in water of at least 20.degree. and a polymer compatible with blood.

### Claim 12 (depends on 11)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 11, wherein said water-insoluble material is an organic polymer.

### Claim 13 (depends on 11)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 11, wherein said water-insoluble material has a contact angle with air bubble in water of at least 40.degree..

### Claim 14 (depends on 11)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 11, wherein said polymer compatible with blood is a polymer having a nitrogen-containing basic functional group.

### Claim 15 (depends on 14)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 14, wherein said polymer having a nitrogen-containing basic functional group is 2-hydroxyethyl methacrylate-diethylaminoethyl methacrylate copolymer.

### Claim 16 (depends on 1)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 1, wherein said water-insoluble carrier is activated using an epoxide.

### Claim 17 (depends on 1)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 1, wherein said water-insoluble carrier is activated using an epichlorohydrin.

### Claim 18 (depends on 1)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 1, wherein said ligand is a collagen electrolyte.

### Claim 19 (depends on 18)

The absorbent for .beta..sub.2 -microglobulin as claimed in claim 18, wherein said collagen electrolyte is methylated atelocollagen Type-I.

### Claim 20 (depends on 1)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 1, wherein said ligand is a gelatin electrolyte.

### Claim 21 (depends on 20)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 20, wherein said gelatin electrolyte is an electrolyte obtained by the heat treatment under an acidic condition of collagen obtained by treating under an acidic condition.

### Claim 22 (depends on 1)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 1, wherein said ligand is a collagen or gelatin electrolyte.

### Claim 23 (depends on 22)

The adsorbent for .beta..sub.2 =microglobulin as claimed in claim 22, wherein said collagen or gelatin electrolyte has molecular weight of from 10,000 to 500,000.

### Claim 24 (depends on 22)

The adsorbent for .beta..sub.2 -microglobulin as claimed in claim 22, wherein said collagen or gelatin electrolyte has an X value for from greater than 2 but not more than 25.

### Claim 25 (depends on 1)

A method for cleaning or regenerating a body fluid derived from patients suffering from renal insufficiency or malignant tumor which comprises contacting said body fluid taken from the body with the adsorbent defined in claim 1, adsorbing and removing .beta..sub.2 -microglobulin at a high efficiency and with specificity, and then returning the cleaned body fluids into the body.

### Claim 26 (depends on 25)

The method as claimed in claim 25, wherein said body fluid is blood, plasma, serum, ascites or hydrothorax.