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AssigneeCalifornia Institute of Technology
InventorsChaitan S. Khosla, James E. Bailey
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The invention relates to nucleotide sequences, including a substantially purified gene which codes for an oxygen-binding protein, and a gene promoter/regulator which is useful in subjecting the translation/transcription of DNA sequences to selective regulation by external control, and plasmid vectors containing those nucleotide sequences, which are valuable bioprocessing catalysts for enhancing the growth characteristics of cells, and increasing production of various proteins and metabolites of those cells. Methods for the use of these nucleotide sequences and related plasmids for a range of applications including oxygen supply to cells, growth enhancement, expression of various gene products, enhancement of oxygen-requiring processes, binding and separation of oxygen from liquids and gases, and a range of oxidative reactions are also disclosed.
A portable DNA sequence comprising the following DNA sequence, (reading 5' to 3', wherein the coding sequence is underlined), or a fragment of said sequence which is capable of acting as a promoter/regulator which is controlled by manipulating environmental oxygen, said fragment including the underlined translation initiator ATG:
The vector pUC19/pRED2.
A portable DNA sequence comprising the coding sequence for the Vitreoscilla hemoglobin protein or for a fragment of said protein having oxygen-binding activity, wherein said Vitreoscilla hemoglobin protein has the following sequence:
A portable DNA sequence comprising the coding sequence for the Vitreoscilla hemoglobin protein or for a fragment of said protein having oxygen-binding activity, wherein the coding sequence of said Vitreoscilla hemoglobin protein is as follows:
A method of preparing an active Vitreoscilla hemoglobin or a fragment of said hemoglobin having oxygen-binding activity, consisting essentially of:
The microorganism Escherichia coli JM101 pUC19/pRED2, having ATCC accession number 67536.
A method for enhancing the growth or yield of products from a microbial cell culture grown in the presence of oxygen, comprising:
Dependencies are inferred from the claim text.