Ceramide-glycanase
OwnerSu-Chen Li, Yu-Teh Liinventors
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Abstract
Ceramide-glycanase which cleaves the entire oligosaccharides intact from glycosphingolipids and alkylglycolipids. The enzyme does not hydrolyze monosaccharides from these glycoconjugates. The enzyme is obtained from the muscle tissue of annelids such as earthworms and leeches by homogenization in water, ammonium sulfate fractionation, gel permeation chromatography, and hydrophobic interaction chromatography, and is essentially free from exo-glycosidase activity. Also disclosed is a method for obtaining oligosaccharides by hydrolysis of glycolipids, alkylglycolipids, and glycosphingolipids by the ceramide-glycanase.
Claims38
A protein composition comprising ceramide-glycanase which functions to hydrolyze the glycosidic linkage of a glycolipid to yield an intact oligosaccharide and a lipid.
A ceramide-glycanase enzyme which functions to hydrolyze the glycosidic linkage of a glycolipid to yield an intact oligosaccharide and a lipid, and which is essentially free of exoglycosidase.
A method for hydrolyzing glycolipids comprising:
- contacting a substrate with ceramide-glycanase essentially free of other glycosidases for a time and under conditions effective to substantially hydrolyze said substrate into products including substantially intact oligosaccharide, said substrate selected from the group consisting essentially of glycoconjugates in which the glycan has at least two saccharides in a chain, the aglycon is hydrophobic and the glycan is glycosidically bonded to the aglycon.
A method for recovering purified ceramide-glycanase from annelids comprising:
- preparing annelid extract in an extraction solvent;
- fractionating proteins in the annelid extract;
- recovering the protein fraction containing ceramide-glycanase;
- and
- purifying said ceramide-glycanase protein by chromatography.
Dependencies are inferred from the claim text.