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AssigneeNoda Institute for Scientific Research
InventorMasaru Suzuki
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A method for measuring creatine in a sample by the use of creatine amidinohydrolase which comprises decomposing the N-ethylglycine present in the sample enzymatically and thereafter reacting sarcosine oxidase upon the sample; and a reagent for use in the measurement of creatine comprising the first reagent and the second reagent, wherein the first reagent comprises a sarcosine oxidase of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 20 mM or below and catalase or comprises said sarcosine oxidase, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatine amidinohydrolase, a sarcosin oxidase of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 50 mM or above, peroxidase and a color reagent for H.sub.2 O.sub.2.
A method for measuring creatine in a sample comprising:
A method for measuring creatinine in a sample comprising decomposing creatine and N-ethylglycine present in the sample enzymatically with creatine amidinohydrolase and sarcosine oxidase of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37.degree. C. is 20 mM or below, and thereafter determining creathinine by reacting the decomposed sample with creatinine amidinohydrolase and sarcosine oxidase N of which Km value (Michaelis constant) to N-ethylglycine at pH 8, 37.degree. C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671) and measuring final reaction products as a measure of creatinine present in the sample.
A reagent for use in the measurement of creatine comprising the first reagent and the second reagent, wherein the first reagent comprises a sarcosine oxidase of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 20 mM or below and catalase or comprises the same sarcosine oxidase as above, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatine amidinohydrolase, sarcosine oxidase N of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671) peroxidase and a color reagent for H.sub.2 O.sub.2.
A reagent for use in the measurement of creatinine comprising the first reagent and the second reagent, wherein the first reagent comprises creatine amidinohydrolasee, a sarconsine oxidase of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 20 mM or below and catalase or comprises the same creatine amidinohydrolase and sarcosine oxidase as above, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatinine amidohydrolase, sarcosine oxidase N of which Km value to N-ethylglycine at pH 8, 37.degree. C. is 50 mM or above as obtained by culturing Bacillus sp. NS-129 (FERM BP-671);
Dependencies are inferred from the claim text.