Method for producing a proteolytic complex stimulating the activity of the pancreas and its application in zootechny

A process for producing an enzymatic complex by fermentation of a strain of Streptomyces fradiae which consists in keeping the pH value within the range of from 7.0 to 7.5 during the culture by adding an inorganic acid or organic acid solution, separating the broth from the mycelium by filtration with a clarifying agent, and fractionating the resulting filtrate by ultrafiltration with a membrane having a cutting threshold corresponding to a molecular weight within the range of from 2,000 to 12,000 and finally atomizing this enzymatic mixture to obtain a proteolytic complex consisting essentially of proteases of type II, III, and IV, in which at least 40% of the proteolytic activity is afforded by protease of type II, and in which the proteases of type III and IV each give at the most 30% of the total proteolytic activity.