Back to results
US 5,045,470 AGranted
Device for submerged culture of tissue cells
AssigneeStawag
InventorGerd Kloss
Life of this patent
Estimating the term…
Abstract
An apparatus for submerged culture of tissue cells in which gas is supplied to the culture medium in an upright sterilizable container through a rotating hollow shaft having a plurality of sets of radially extending blades from which the gas is discharged only along upper surfaces of the blades within a cage formed by vertically spaced hollow baffle plates connected by hollow posts through which gas is supplied to the baffle plates and is charged into the nutrient medium through porous parts of the plates.
Claims5
An apparatus for submerged culturing of tissue cells, comprising:
- an upright sterilizable container;
- a rotatable vertical hollow shaft extending centrally in said container;
- a plurality of sets of radially extending blades spaced apart on said shaft and communicating with said hollow shaft, each of said blades being permeable to gas only along an upper surface thereof whereby gas introduced into said shaft flows out of said upper surfaces of said blades into a culture medium in said container, each of said sets comprising at least one pair of said blades, said blades having gas-impermeable lower surfaces;
- spacers between said sets along said shaft;
- means for feeding said gas to said hollow shaft;
- and
- a cage in said container surrounding said shaft and said sets of blades said cage comprising:
- a plurality of vertically spaced hollow baffle plates each disposed between two of said sets,
- hollow posts outwardly of said blades attached to said plates and communicating with said plates,
- means for feeding gas to said hollow posts, and
- gas-permeable means on said plates for discharging gas into said medium.
8 more elements
Dependencies are inferred from the claim text.