Column analyzer system
AssigneeHelena Laboratories Corporation
InventorsJames R. M. Sanford, Patrick M. Frank, Joseph H. Golias, William C. Jennings
Life of this patent
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Abstract
An analyzer system for automatic column chromatography, and method for its use, includes an array of chromatograph columns and multi-cell cuvettes associated with each column. Chromatographic separation takes place under a constant, low fluid pressure. A pressure system distributes air to each column during chromatographic separation but prevents leakage of air if the column array is partially empty. The multi-cell cuvette collects and separates the eluates associated with a single column. The system provides for automatic removal of caps from the bottoms of the chromatograph columns and provides for automatic optical density reading.
Claims19
A system for delivering a constant low pressure to a chromatography column positioned within means for holding an array of chromatography columns comprising:
- a manifold adapted to be connected to a source of low pressure fluid and adapted to be connected to cylinder means having first and second positions and having a fluid path therethrough;
- said first position for preventing fluid from said manifold from entering the fluid path of said cylinder means;
- said second position for providing fluid flow from said manifold through the fluid path of said cylinder means;
- said cylinder means adapted to engage a chromatographic column in a fluid sealing relationship;
- and
- said cylinder means being normally in said first position and being movable into said second position by contact with said chromatographic column;
- said second position of said cylinder means thereby allowing said constant low pressure provided to said manifold system to be delivered to said chromatography column.
A method for delivering a constant low pressure to a chromatography column positioned within means for holding an array of chromatography columns comprising:
- providing a manifold adapted to be connected to a source of low pressure fluid;
- providing a pressure cylinder coupled to said manifold and having first and second positions and having a fluid path therethrough;
- said first position for preventing fluid from entering the fluid path of said cylinder;
- said second position for providing fluid flow from said manifold through the fluid path of said cylinder;
- said cylinder being normally in said first position;
- and
- moving said cylinder from said first position into said second position by contact with a chromatographic column;
- said second position of said cylinder means thereby allowing said constant low pressure provided to said manifold system to be delivered to said chromatography column.
Dependencies are inferred from the claim text.