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US 5,043,062 AGranted
High performance affinity chromatography column comprising non-porous, nondisperse polymeric packing material
AssigneeEastman Kodak Company
InventorsMarsha D. Bale, Richard C. Sutton
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Abstract
A high performance affinity chromatography separation device comprising: A) a chromatographic column containing: B) a packing material; wherein the packing material is a plurality of non-porous, nonodisperse polymeric particles having i) a particle size in the range of 0.01 to about 5 micrometers and ii) surface reactive groups which are directly or indirectly reactive with free amino groups, sulfhydryl groups, carboxy groups or aldehyde groups of biological ligands is disclosed.
Claims6
A high performance affinity chromatography separation device comprising:
- A) a chromatographic column containing:
- B) a packing material;
- wherein the packing material is a plurality of non-porous, monodisperse polymeric particles having i) a particle size in the range of 0.01 to about 5 micrometers and ii) a surface reactive group having the formula:
- ##STR3## wherein R is hydrogen or substituted or unsubstituted alkyl;
- R.sup.1 is --CH.dbd.CHR.sup.2 or --CH.sub.2 CH.sub.2 X wherein X is a leaving group which is displaced by a nucleophile or is eliminated in the form of HX by treatment with a base;
- R.sup.2 is hydrogen, substituted or unsubstituted alkyl of 1 to 6 carbon atoms, or substituted or unsubstituted aryl of 6 to 12 nuclear carbon atoms;
- L is a substituted or unsubstituted alkylene linking group having 1 to 20 carbon and hetero atoms in the backbone;
- substituted or unsubstituted arylene having 6 to 12 nuclear carbon atoms or a divalent combination of one or more of each of the alkylene and arylene groups defined above.
5 more elements
Dependencies are inferred from the claim text.