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US 5,037,762 AGranted
Water-compatible reducible compounds and their use in analytical compositions and methods
AssigneeEastman Kodak Company
InventorsAlbert J. Mura, Robert T. Belly, Vanessa R. Lum
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Abstract
Certain water-compatible reducible compounds are useful in analytical compositions and elements for assay of various analytes, e.g. microorganisms. These compounds comprise a moiety which provides a detectable species (e.g. a dye) when released from the compound at physiological pH. Further, these compounds are aromatic derivatives or quinones having water-compatibilizing substituent which allow them to be used in compositions without the use of surfactants.
Claims5
A method for the determination of an analyte comprising the steps of:
- A. contacting a sample of a liquid suspected of containing an analyte with a water-compatible reducible compound of the structure CAR--R.sup.1).sub.n wherein CAR- is a substituted or unsubstituted aromatic or quinone nucleus, R.sup.1 is a moiety which comprises a shiftable detectable species, and n is 1 or 2,
- provided said compound is constructed such that it is capable of being reduced at said pH to release said shiftable detectable species, and said compound comprises at least one water-compatibilizing moiety which has a hydrophobic parameter less than about -2.0, and
- further provided that when R.sup.1 is replaced with H, CAR--H).sub.n is constructed such that it has an E.sub.1/2 of at least about +100 mV when measured in water, and
- B. detecting the detectable species released as a result of the presence of said analyte.
1 more element
Dependencies are inferred from the claim text.