BAR1 secretion signal

A yeast cell transformed with a DNA construct comprising the following operably linked elements:

• a transcriptional promoter;
• a first DNA sequence encoding a signal peptide;
• a second DNA sequence encoding at least a portion of the C-terminal domain of the BAR1 gene product capable of directing the export of heterologous proteins when used in combination with a signal peptide;
• and
• a third DNA sequence encoding a heterologous protein or polypeptide.

A mammalian cell transformed with a DNA construct comprising the following operably linked elements:

• a transcriptional promoter;
• a first DNA sequence encoding a signal peptide;
• a second DNA sequence encoding at least a portion of the C-terminal domain of the BAR1 gene product capable of directing the export of heterologous proteins when used in combination with a signal peptide;
• and
• a third DNA sequence encoding a heterologous protein or polypeptide.

A method of producing a secreted protein of interest, comprising:

• growing a host cell containing a DNA construct comprising the following operably linked elements:
• a transcriptional promoter;
• a first DNA sequence encoding a signal peptide;
• a second DNA sequence encoding at least a portion of the C-terminal domain of the BAR1 gene product capable of directing the export of heterologous proteins when used in combination with a signal peptide;
• and
• a third DNA sequence encoding a heterologous protein or polypeptide in an appropriate medium;
• and
• isolating the protein or polypeptide product from said medium.