Endothelial cell procurement and deposition kit

A method for sterilely procuring and depositing endothelial cells onto a graft to be implanted in a patient, said method comprising the steps of:

• providing a kit for collecting fat from a patient, processing said fat to produce an endothelial cell deposition product, and depositing said product on the surface of a graft, all under sterile conditions established and maintained within the components of said kit including a fat collection means for collecting fat from a patient, a digestion means for producing a digested product, an endothelial cell means for producing an endothelial cell product and a cell deposition means for depositing said endothelial cell product on said graft;
• collecting fat from a patient into said sterile fat collection means;
• sterilely transferring said collected fat into said sterile digestion means;
• rinsing and digesting said fat within said digestion means to produce a digested product;
• sterilely transferring said digested product into said sterile isolation means;
• isolating endothelial cells from said digested product within said isolation means to produce an endothelial cell product;
• sterilely transferring said endothelial cell product into said sterile cell deposition means;
• and
• sterilely depositing said cell product onto the surface of the graft to be implanted into a patient.