Chimeric genes suitable for expression in plant cells
AssigneeMonsanto Company
InventorsStephen G. Rogers, Robert T. Fraley
Life of this patent
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Abstract
This invention relates to chimeric genes which are capable of being expressed in plant cells. Such genes contain (a) a promoter region derived in a gene which is expressed in plant cells, such as the nopaline synthase gene; (b) a coding or structural sequence which is heterologous with respect to the promoter region; and (c) an appropriate 3' non-translated region. Such genes have been used to create antibiotic-resistant plant cells; they are also useful for creating herbicide-resistant plants, and plants which contain mammalian polypeptides.
Claims31
A chimeric gene capable of expressing a polypeptide in plant comprising in sequence:
- a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1.5-bis-phosphate carboxylase small subunit gene;
- b) a structural DNA sequence encoding a polypeptide that permits the selection of transformed plant cells containing said chimeric gene by rendering said plant cells resistant to an amount of an antibiotic that would be toxic to non-transformed plant cells, said structural DNA sequence being heterologous with respect to the promoter region;
- and
- c) a 3' non-translated region of a gene naturally expressed in plants, said region encoding a signal sequence for polyadenylation of mRNA.
A chimeric gene comprising in sequence:
- (a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1.5-bis-phosphate carboxylase small subunit gene;
- (b) a heterologous structural DNA sequence encoding a neomycin phosphotransferase;
- and
- (c) a 3' non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.
A microorganism identified by ATCC Accession Number 39264.
Dependencies are inferred from the claim text.