# US 5,032,247 A · Method and apparatus for electrophoretic separations

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- Number: US 5,032,247 A
- Document ID: US5032247A
- Status: Granted
- Kind: A
- Application number: 07/407,173
- Filed: 1989-09-14
- Granted: 1991-07-16
- Earliest claimed: 1989-09-14
- Assignees: Separations Technology, Inc.
- Inventors: Yuri Tarnopolsky
- Web page: https://patentgrep.com/patent/US5032247A

## CPC

- G01N 27/44708: {Cooling}
- G01N 27/44769: {Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A method and apparatus for conducting electrophoretic separations is described in which heat exchange fluid is passed through hollow fibers in the electrophoretic chamber during the separation process and the separations are conducted in the interstices of the hollow fiber. Hollow fibers made of organic polymers, inorganic materials such as glass and silica, and natural fibers may be used.

## Claims

### Claim 1

A system for conducting an electrophoretic separation comprising, an electrophoresis chamber having an anode and a cathode positioned across the chamber to provide current flow through said chamber, a plurality of hollow fibers packed in said chamber and passing through the chamber, said fibers having inlets and outlets, means to introduce heat exchange fluid through said fibers, means at one end of said chamber to introduce a solution to be separated, means to remove solution from the opposite end of the chamber in a plurality of streams, the packing of the fibers being arranged to provide sufficient space therebetween to permit the solution to be separated to flow from one end of the chamber to the other in the interstices provided between the fibers by the packing.

### Claim 2 (depends on 1)

The system of claim 1 including spacers located between the fibers in the horizontal direction across said chamber, said spacers being permeable to fluid flow.

### Claim 3 (depends on 1)

The system of claim 1 wherein spacers are provided between the fibers in the vertical direction in the chamber, the spacers being permeable to fluid flow.

### Claim 4 (depends on 1)

The system of claim 1 wherein spacers are provided between the fibers in the horizontal and vertical directions in the chamber, the spacers being permeable to fluid flow.

### Claim 5 (depends on 2)

The system of claim 2 wherein the spacer is selected from the group consisting of cloth, mats, scrims, meshes, filaments, permeable membranes and papers.

### Claim 6 (depends on 3)

The system of claim 3 wherein the spacer is selected from the group consisting of cloth, mats, scrims, meshes, filaments, permeable membranes and papers.

### Claim 7 (depends on 4)

The system of claim 4 wherein the spacer is selected from the group consisting of cloth, mats, scrims, meshes, filaments, permeable membranes and papers.

### Claim 8 (depends on 1)

The system of claim 1 wherein the fibers having external diameters of 10 to 500 microns.

### Claim 9 (depends on 1)

The system of claim 1 wherein means to introduce a second fluid to the interstices between the fibers is provided in said chamber.