Receptors for efficient determination of ligands and their antagonists or agonists
AssigneeGenentech, Inc.
InventorsThomas J. Dull, Heimo Riedel, Axel Ullrich
Life of this patent
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Abstract
Hybrid receptors are provided that comprise (a) the ligand binding domain of a predetermined receptor and (b) a heterologous reporter polypeptide. The hybrid receptors are useful for convenient and large scale assay of biologically active ligands or their antagonists or agonists.
Claims7
Nucleic acid encoding a hybrid receptor for a ligand comprising (a) the ligand binding domain of a predetermined receptor and (b) a heterologous reporter polypeptide which undergoes a conformational change upon the binding of sasid ligasnd to the ligand binding domain.
A method formasking a hybrid receptor for a ligand which receptor comprises (1) the ligand binding domasin of a predetermined receptor and (2) a heterologous reporter polypeptide which undergoes a conformational change upon the binding of said ligand to the ligand binding domsain, sasid method comprising
- (a) transforming a host cell with a vector containing nucleic acid encoding the hybrid receptor operably linked to a promoter for controlling the transcription of the hybrid receptor;
- and
- (b) culturing the host cell under conditions for expressing the hybrid receptor.
Dependencies are inferred from the claim text.