Enzymatic peracid bleaching system
AssigneeThe Clorox Company
InventorsRichard J. Wiersema, Anna G. Stanislowski, Gregory L. Gray, Ayrookaram J. Poulose, Scott D. Power
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Abstract
An enzymatic perhydrolysis system, useful for bleaching, has a novel enzyme, a substrate, and a source of hydrogen peroxide, and provides in situ formation of peracid in aqueous solution. The substrate is selected for enzyme catalyzed reaction, and preferably is an acylglycerol with two or three fatty acid chains. The enzyme is hydrolytically and perhydrolytically active even in the presence of anionic surfactants, has lipase activity, and is isolatable from Pseudomonas putida ATCC 53552.
Claims16
An enzymatic perhydrolysis composition for in situ generation of peracid comprising:
- (a) an enzyme having hydrolase activity and being isolated or cloned from Pseudomonas putida ATCC 53552 or genetic variants thereof, the enzyme being resolvable as a single band by SDS electrophoresis and having a molecular weight of about 30,000 daltons;
- (b) a substrate being capable of hydrolysis by the enzyme of (a), the substrate including a glyceride, an ethylene glycol derivative or a propylene glycol derivative, the glyceride being one or more of a monoglyceride with a fatty acid chain of one to about twelve carbons, a diglyceride with fatty acid chains of one to about twelve carbons, and a triglyceride with fatty acid chains of one to about twelve carbons, the ethylene glycol derivative having the structure ##STR5## where n=1-10 and where ##STR6## is the acyl moiety of a fatty acid and R.sub.1 has one to about twelve carbons, and the propylene glycol derivative having the structure ##STR7## where n and R.sub.1 are defined above;
- and (c) a source of peroxygen which will react with (a) and (b) to produce peracid in the presence of a substrate-suspending aqueous solution.
A process for bleaching materials, comprising the steps of contacting the materials with an aqueous solution and combining with the aqueous solution an enzymatic perhydrolysis composition for in situ generation of peracid, said composition including:
- (a) an enzyme isolated from an organism expressing a coding region found in or cloned from Pseudomonas putida ATCC 53552, the enzyme having a molecular weight of about 30,000 daltons and being resolvable as a single band by SDS gel electrophoresis;
- (b) a substrate being capable of hydrolysis by the enzyme of (a), the substrate including a glyceride, an ethylene glycol derivative or a propylene glycol derivative, the glyceride being one or more of a monoglyceride with a fatty acid chain of one to about twelve carbons, a diglyceride with fatty acid chains of one to about twelve carbons, and a triglyceride with fatty acid chains of one to about twelve carbons, the ethylene glycol derivative having the structure ##STR8## where n=1-10 and where ##STR9## is the acyl moiety of a fatty acid and R.sub.1 has one to about twelve carbons, and the propylene glycol derivative having the structure ##STR10## where n and R.sub.1 are defined above;
- and (c) a source of peroxygen which will react with (a) and (b) to produce paracid.
Dependencies are inferred from the claim text.