Transient expression system for producing recombinant protein
AssigneeGenentech, Inc.
InventorCornelia M. Gorman
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Abstract
A method is described for transient production of a desired heterologous protein comprising: transfecting a eukaryotic host cell with a vector producing a trans-activating protein; transfecting the eukaryotic host cell with an expression vector comprising a stabilizing sequence downstream of a promoter and upstream of a DNA encoding the desired heterologous protein and a polyadenylation sequence downstream of which is a transcription terminatein site; culturing the transfected eukaryotic host cell under conditions favorable for production of said desired heterologous protein; and, recovering the desired protein in useful amounts within about one day to about fourteen days of transfection.
Claims27
A method for the enhanced transient expression of a desired protein in a host kidney cell comprising:
- a) providing a host kidney cell which expresses an adenovirus trans-activating protein;
- b) transfecting said host kidney cell with a vector comprising DNA encoding the desired protein under the control of a cytomegalovirus promoter;
- and,
- c) culturing said host kidney cell for a period comprising 1 to 14 days under conditions favorable for transient production of the desired protein.
A host kidney cell wherein said host kidney cell endogenously produces an adenovirus trans-activating protein and is transformed by transfecting said host kidney cell with a vector comprising DNA encoding said protein under the control of a cytomegalovirus promoter.
In a method for production of a host kidney cell capable of production of a desired heterologous protein, wherein said host kidney cell endogenously produces an adenovirus trans-activating protein, the improvement comprising transfecting said host kidney cell with a vector comprising DNA encoding said desired protein under the control of a cytomegalovirus promoter.
Dependencies are inferred from the claim text.