Detection of necrotic malignant tissue and associated therapy

A method for delivering primary, de novo therapy to a wide variety of cancer types, both primary tumors and metastases, to kill neoplastic cells in vivo in a mammal, comprising the steps of:

• obtaining an antibody specific to an insoluble internal cellular antigen, wherein said antibody is obtained by screening a library of antibodies generated to insoluble intracellular antigens, and selecting those antibodies that are specific to insoluble intracellular antigen present in both neoplastic and normal cells but not present on the exterior of living cells and that is not released into the general circulation upon cell death, wherein said antibody has directly or indirectly bound thereto an antineoplastic agent;
• and
• initiating therapy directly by administering said antibody-antineoplastic agent conjugate to said mammal, whereby said antibody conjugate becomes selectively bound to permeable cells present within primary or metastatic neoplasms in said mammal, thereby delivering said antineoplastic agent to cells surrounding said permeable cells.

A monoclonal antibody to an insoluble intracellular antigen present in cells which may be induced to be permeable, or in cell ghosts of substantially all neoplastic and normal cells, but not present on the exterior of living cells of said mammal, wherein said antibody is bound to a moiety comprising at least in part an alpha-emitting radionuclide or a beta-emitting radionuclide.

A monoclonal antibody to an insoluble intracellular antigen present in cells which may be induced to be permeable, or in cell ghosts of substantially all neoplastic and normal cells of a mammal, but not present on the exterior of living cells of said mammal, wherein said antibody is bound to a moiety comprising at least in part a chemotherapeutic or immunotherapeutic agent or a biological response modifier.