Concentration determination with multiple wavelength flash photometers

A monobeam system of determining the concentration of at least one chromophore substance in a sample comprising a lamp for flashing a light beam through the sample, means for dispersing the light beam passing through the sample into multiple wavelengths, means for obtaining the intensity of the signal of the light beam at selected multiple wavelengths, means for determining from the intensity values the absorbance values as different wave-lengths, and means for determining the absorbance values at two or more wavelengths thereby to obtain a measure of the chromophore concentration, and including a monochromator for dispersing the light beam from a flash lamp, a lens for diverging the light beam from the flash lamp, the monochromator including a first collimator mirror for developing, from the diverging beam, a collimated beam directed towards a diffraction grating, the grating dispersing the collimated beam according to wavelength, a second collimator mirror receiving the multiple beams and reflecting the multiple beams into convergent beams at selected positions at a plane, the plane being located at a preselected plane for different wavelengths of the convergent beams.

A method of determining the concentration of at least one chromophore substance in a sample in a monobeam system comprising flashing a light beam through the sample, dispersing the light beam passing through the sample into multiple wavelengths, obtaining the intensity of the signal of the light beam at selected multiple wave-lengths, determining from the intensity values the absorbance values at different wavelengths, and determining absorbance values at two or more wavelengths thereby to obtain a measure of the chromophore concentration, and including diverging the light beam from the flash lamp, directing the diverging light beam to a first collimator mirror for developing, from the diverging beam, a collimated beam, directing the collimated beam towards a diffraction grating, the grating dispersing the collimated beam according to wavelength into multiple beams, directing the beams to a second collimator mirror and reflecting the multiple divergent beams into convergent beams directed at selected positions at a plane, and processing the intensity of the light beam at the plane at different wavelengths to determine absorbance as a measure of concentration.

A monobeam system comprising a lamp for flashing a light beam through a sample, means for dispersing the light beam passing through the sample into multiple wavelengths, and means for obtaining the intensity of the signal of the light beam at selected multiple wavelengths, and including a monochromator for dispersing the light beam from a flash lamp, optics for directing the light beam from the flash lamp, the monochromator including a first collimator mirror for developing, from the diverging beam, a collimated beam directed towards a diffraction grating, the grating dispersing the collimated beam according to wavelength, and a second collimator mirror receiving the multiple beams and reflecting the multiple beams into convergent beams at selected positions at a plane, the plane being located at a predetermined position for different wavelengths of the convergent beams.

Apparatus operationally connected with an output detector photoarray of a monochromator from a monobeam system, the photoarray being adapted to receive, in analog form, an intensity signal of selected wavelengths of the beam related to a sample in a path of the beam, and comprising means for processing the analog intensity signals to obtain digital absorbance signal data and including processor means for processing the digital data to obtain a linear combination of absorbance values at the different wavelengths, and means for determining consequent concentration values from the absorbance values.

A method of determining an optimum of absorbance values at different wavelengths of a light beam passing through a sample chromophore substance in a sample comprising:

• (a) flashing a lamp to generate a flashing light beam to pass through the sample containing the chromophore substance;
• and
• (b) measuring the absorbance values by the sample at different wavelengths, wherein errors in absorbance values are at different optimum wavelengths.