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AssigneeE. I. Du Pont de Nemours and Company
InventorsJohn R. Bedbrook, Roy S. Chaleff, Saverio C. Falco, Barbara J. Mazur, Christopher R. Somerville, Narendra S. Yadav
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A nucleic acid fragment encoding a herbicide-resistant plant acetolactate synthase protein is disclosed. This nucleic acid fragment contains at least one nucleotide mutation resulting in one amino acid change in one of seven substantially conserved regions of acetolactate synthase amino acid homology. This mutation results in the production of an acetolactate synthase protein which is resistant to sulfonylurea herbicide compounds compared to the wild-type protein. Transformation of herbicide sensitive plants or plant cells with the fragment results in resistance to the herbicide.
An isolated nucleic acid fragment comprising a nucleotide sequence encoding a plant acetolactate synthase protein which is resistant to a compound selected from the group consisting of sulfonylures, triazolopyrimidine sulfonamide, and imidazolinone herbicides, said nucleotide sequence comprises at least one sub-sequence which encodes one of the substantially conserved amino acid sub-sequences designated A, B, C, D, E, F, and G is FIG. 6, the nucleic acid fragment is further characterized in that at least one of the following conditions is met,
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