Hybridoma and monoclonal antibody for use in an immunoaffinity purification system
AssigneeImmunex Corporation
InventorsThomas P. Hopp, Kathryn S. Prickett
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Abstract
A particular calcium-dependent monoclonal antibody, 4E11, is produced from a murine hybridoma cell line and is useful for purifying a recombinant fusion protein having an N-terminal identification peptide DYKDDDDK to which the monoclonal antibody binds.
Claims4
Murine hybridoma 4E11 (ATCC HB 9259).
A monoclonal antibody having essentially the same binding affinity and epitopic specificity of a monoclonal antibody secreted by murine hybridoma 4E11 (ATCC HB 9259).
A monoclonal antibody that binds the peptide DYKDDDDK only in the presence of a divalent metal cation selected from the group consisting of Ca.sup.++, Mg.sup.++, Mn.sup.++, Cu.sup.++, Ni.sup.++, Zn.sup.++, Cd.sup.++, and.
Dependencies are inferred from the claim text.