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AssigneeThe Regents of the University of California
InventorsWilliam J. Rutter, Daniel V. Santi
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A method to obtain selected individual peptides or families thereof which have a target property and optionally to determine the amino acid sequence of a selected peptide or peptides to permit synthesis in practical quantities is disclosed. In general outline, the method of the invention comprises synthesizing a mixture of randomly or deliberately generated peptides using standard synthesis techniques, but adjusting the individual concentrations of the components of a mixture of sequentially added amino acids according to the coupling constants for each amino acid/amino acid coupling. The subgroup of peptides having the target property can then be selected, and either each peptide isolated and sequenced, or analysis performed on the mixture to permit its composition to be reproduced. Also included in the invention is an efficient method to determine the relevant coupling constants.
A method of preparing in the same reaction vessel a mixture of peptides of distinct, unique and different sequences which mixture contains each peptide in retrievable and analyzable amounts and in substantially equal molar amounts, comprising:
A method of obtaining a peptide or mixture of peptides of a specified target property, which method comprises the steps of:
A method of preparing a mixture of peptides containing a recoverable amount of a peptide having a specified target property, comprising:
The method of preparing a mixture of peptides of distinct, unique and different sequences containing a peptide of a desired amino acid sequence and a specified target property;
A method of determining the sequence of a peptide containing at least fifty peptide in a mixture of peptides wherein the mixture of peptides resulted from a reaction of distinct, unique and different sequences or set of reactions which produced each peptide in the mixture in substantially equal molar amounts and subjecting the mixture to a battery of analytical techniques to obtain analytical data which is manipulated to determine the sequence of individual peptides.
Dependencies are inferred from the claim text.