Ascorbic acid intermediates and process enzymes
AssigneeGenentech, Inc.
InventorsStephen Anderson, David R. Light, Cara Marks, William H. Rastetter
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Abstract
Methods for producing ascorbic acid using recombinant means comprising the transfer of genetic material by conjugation, a host cell lacking, entirely or to such an extent as not to be commercially useful, one or more enzymes in the metabolic path converting glucose to 2 keto-L-gulonic acid.
Claims12
A process for converting glucose to 2-KLG which process comprises culturing in a medium containing glucose under suitable metabolic conditions, bacterial cells to which genetic material has been transferred wherein the transfer of said genetic material renders a bacterial host cell capable of converting glucose to 2,5-DKG but lacking the ability to convert 2,5-DKG to 2-KLG, capable of converting 2,5-DKG into 2-KLG.
A microorganism to which genetic material encoding 2,5-DKG reductase is transferred such that said transfer renders a host cell capable of converting glucose to 2,5-DKG but lacking the ability to convert 2,5-DKG to 2-KLG, capable of effecting the conversion of 2,5-DKG into 2-KLG.
Dependencies are inferred from the claim text.