Vectors and host cells expressing prepromotilin and motilin-associated peptide (MAP)
AssigneeOregon Health Sciences University
InventorsJohn P. Adelman, Chris T. Bond, Gajanan Nilaver, Earl A. Zimmerman
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Abstract
cDNA encoding a precursor peptide for motilin has been retrieved from porcine gut tissue. The precursor protein contains a motilin-associated peptide (MAP) sequence conjugated to the motilin peptide through a trypsin-like cleavage site. The MAP is useful in regulating alimentary physiology in vertebrates. The DNA provides a means to obtain the corresponding DNAs in other vertebrate species, including humans. It also makes available recombinant motilin, motilin precursor, and MAP of any desired species.
Claims4
A recombinant expression vector comprising control sequences operably linked to a DNA encoding a protein functionally equivalent to motilin precursor or preprotein forms thereof, said DNA including a nucleotide sequence substantially equivalent to the sequence depicted in FIG. 1 encoding for amino acid residues 1-94.
A recombinant expression vector comprising control sequences operably linked to a DNA encoding a protein functionally equivalent to MAP or preprotein forms thereof, said DNA including a nucleotide sequence substantially equivalent to the sequence depicted in FIG. 1 encoding for amino acid residues 25-94.
Dependencies are inferred from the claim text.