Hepatocyte growth factor
AssigneesShuji Hashimoto, Yasushi Daikuhara, Eiichi Gohda, Hirohito Tsubouchi, Mitsubishi Kasei Corporation
InventorsEiichi Gohda, Hirohito Tsubouchi, Hiroyuki Nakayama, Shuichi Hirono, Kozo Takahashi, Shuji Hashimoto, Yasushi Daikuhara
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Abstract
A hepatocyte growth factor comprising a proteinous substance derived from human blood and showing the following physicochemical properties and physiologic activity: (i) its molecular weight is estimated to be approximately 76,000 to 92,00 by SDS-PAGE analysis (under non-reductive conditon); (ii) it possesses HGF activity; (iii) the HGF activity is inactivated by heat treatment for 10 minutes at 80.degree. C.; (iv) the HGF activity may be lost by digestion with trypsin or with chymotrypsin; and (v) it has a high affinity for heparin.
Claims3
A substantially purified and isolated human hepatocyte growth factor, comprising a proteinaceous substance which exhibits the following physicochemical properties and physiological activity:
- i) having a molecular weight which is estimated to be approximately 76,000-92,000 by SDS-PAGE analysis, under non-reductive conditions;
- (ii) having HGF activity;
- (iii) said HGF activity being inactivated by heat treatment for 10 minutes at 80.degree. C.;
- (iv) said HGF activity being lost by digestion with trypsin or with chymotrypsin;
- and
- (v) having such a high affinity for heparin that when adsorbed by Heparin-Sepharose column, said growth factor is not eluted therefrom below 0.8M NaCl in a PBS(-) buffer at pH 7.4 containing 0.013% Triton X-100.
Dependencies are inferred from the claim text.