Cloning and expression of phosopholipase C genes
AssigneeProtein Design Labs, Inc.
InventorsJ. Yun Tso, Cary L. Queen
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Abstract
Improved means for producing Clostridium Phospholipase C (PL) polypeptides based on the cloning and expression of recombinant DNA segments containing Clostridium PLC genes and fragments. The DNA segments are operably linked to host specific expression control sequences for exogenous production of Clostridium PLC, or fragments thereof, substantially free from naturally-associated Clostridium gene products.
Claims18
An isolated recombinant DNA segment encoding a Clostridium Phospholipase C polypeptide, said DNA segment comprising a first DNA sequence selected from the group consisting of:
- (a) the DNA sequences of FIGS. 1 and 2, or their complementary strands;
- (b) DNA sequences which on expression code for the polypeptide sequences of FIGS. 1 and 2, or the complementary strands of said DNA sequences;
- and
- (c) DNA sequences which hybridize under stringent conditions to sequences of (a) or (b).
A recombinant vector which, in a transformed or transfected host, will express a DNA segment coding for a Clostridium Phospholipase C polypeptide.
A process for producing a Clostridium Phospholipase C polypeptide comprising the steps of:
- (a) forming a vector comprising a nucleotide sequence coding for said polypeptide, said sequence operably linked to an expression control sequence;
- (b) transforming or transfecting a host cell with the vector;
- and
- (c) maintaining the host cell under conditions suitable for expression of the nucleotide sequence.
A cloned gene coding for a Clostridium Phospholipase C, wherein said gene is substantially free from naturally associated Clostridium genes.
Dependencies are inferred from the claim text.