Method for ATP extraction
AssigneeLumac BV
InventorsWilliam J. Simpson, John R. M. Hammond
Life of this patent
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Abstract
A method for the extraction of ATP from a microorganism which comprises contacting said microroganism with an ATP releasing agent and thereafter contacting the resultant solution with a neutralizing agent which acts substantially to eliminate the distorting effect of the releasing agent on subsequent ATP assay.
Claims5
In a method for the assay of ATP from a microorganism which comprises contacting a sample containing said microorganism with an ATP releasing agent consisting essentially of an effective amount to release ATP of a cationic surface active agent, and thereafter determining the ATP released into the sample using a luciferin-luciferase reagent, the improvement which comprises eliminating the distorting effect of the releasing agent on the ATP assay, by addition of an amount of a non-ionic surface active agent to be effective as a neutralizing agent for the releasing agent after addition of said releasing agent and before said ATP assay.
Dependencies are inferred from the claim text.