Process for photometric determination of protein C and/or protein S
AssigneeBoehringer Mannheim GmbH
InventorsKnut Bartl, Andreas Dessauer, Helmut Lill
Life of this patent
Estimating the term…
Abstract
The present invention provides a process for the photometric determination of protein C and/or protein S activity, especially in plasma, wherein the sample containing the protein C and/or protein S to be determined is incubated with a protein C activator from snake venom with the formation of activated protein C and/or protein S and the decrease of the formation of thrombin from prothrombin brought about by the coagulation factors and the activators thereof is determined by means of a chromogenic thrombin substrate.
Claims13
Method for photometric determination of at least one of active protein C and active protein S in a sample comprising simultaneously incubating the sample with a protein C activator from snake venom which activates protein C and/or protein S, a chromogenic thrombin substrate and a coagulation system activator and determining cleavage of said chromogenic thrombin substrate by thrombin as an indicator of active protein C and/or protein S in said sample, without adding an inactivator of said protein C activator.
Dependencies are inferred from the claim text.