Method for x and y spermatozoa separation
AssigneeUniversity of Kentucky Research Foundation
InventorsPanayiotis M. Zavos, Karl A. Dawson
Life of this patent
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Abstract
A method for separating male and female determining spermatozoa includes the initial step of exposing freshly ejaculated spermatozoa in a substantially protein free diluent to an excess concentration of a monoclonal antibody directed against H-Y antigen that binds substantially exclusively with male determining spermatozoa. The method continues with the suspending of the exposed spermatozoa together with a conjugate of (1) an immunoglobulin G antibody that binds substantially exclusively to the monoclonal antibody and (2) an immunoabsorbant substrate in a substantially protein free diluent. This forms a conjugate/spermatozoa preparation. The method concludes with the recovering of the separated male and female determining spermatozoa.
Claims17
The method for separating male and female determining spermatozoa for the purpose of controlling the sex of offspring prior to conception, comprising the steps of:
- exposing the spermatozoa in a protein free diluent to an excess concentration of a first antibody that binds male determining spermatozoa;
- suspending said exposed spermatozoa together with a conjugate of a second antibody that binds exclusively to said first antibody and an immunoabsorbent substrate in a protein free diluent to form a conjugate/spermatozoa preparation;
- and
- recovering the female determining spermatozoa from the conjugate/spermatozoa preparation while said male determining spermatozoa are bound to said immunoabsorbent substrate.
Dependencies are inferred from the claim text.