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US 4,994,384 AGranted
Multiplying bovine embryos
AssigneeW. R. Grace & Co.-Conn.
InventorsRandall S. Prather, Frank Barnes, James Robl, Neal L. First, Vincent F. Simmon
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Abstract
A method of multiplying bovine embryos by transfer of nuclei is described. The method includes enucleating a recipient oocyte, transferring the donor embryo nucleus to the enucleated recipient oocyte, and fusing the donor nucleus to the recipient oocyte by dielectrophoresis. The method is intended to allow the generation of multiplied genetically identical cattle.
Claims23
A method for transplanting a nucleus from a two to thirty-two cell donor bovine embryo to a recipient oocyte comprising the steps of:
- (a) isolating a membrane-bounded nucleus from a cell of the donor embryo;
- (b) removing the nuclear chromosomal material from the recipient oocyte (to prepare an enucleated recipient oocyte);
- (c) placing the donor membrane-bounded nucleus with its membrane adjacent to that of the enucleated recipient oocyte;
- and
- (d) electrically fusing the membranes of the membrane-bounded nucleus and the enucleated recipient oocyte together to form an embryonic single cell with a nucleus from the donor embryo.
2 more elements
A method for producing cloned bovine embryos comprising:
- (a) isolating a nucleus, bounded by a membrane, from a single cell of a two to thirty-two cell donor bovine embryo;
- (b) enucleating a recipient oocyte;
- (c) introducing the donor membrane-bounded nucleus into the perivitelline space of the enucleated recipient oocyte;
- (d) electrically inducing cell fusion between the membrane bounding the donor nucleus and the enucleated recipient oocyte;
- (e) culturing in vitro the electrically induced fused embryo obtained in step (d);
- and
- (f) transferring the cultured embryo of step (e) to the oviduct of a recipient maternal animal.
4 more elements
Dependencies are inferred from the claim text.