Method and structures employing chemically-labelled polynucleotide probes
AssigneeEnzo Biochem, Inc.
InventorsJannis G. Stavrianopoulos, Dollie Kirtikar, Kenneth H. Johnston, Barbara E. Thalenfeld
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Abstract
Polynucleotide sequences in a sample of biological or nonbiological material are detected by a method involving fixing of the sequences on a solid support and forming an entity between the fixed sequences and chemically-labeled polynucleotide or oligonucleotide probes having a sequence complementary to the fixed sequence for determining the identification and/or presence of the target polynucleotide sequences. The chemical label covalently or noncovalently attached to the probe comprises a signalling moiety capable of generating a soluble signal detectable by spectrophotometric assay techniques.
Claims27
A method for detecting a polynucleotide sequence which comprises:
- fixing said polynucleotide sequence to a solid support which comprises or is contained within a transparent or translucent, non-porous system, such that a single-strand of the polynucleotide is capable of hybridizing to complementary nucleic acid sequences;
- forming an entity comprising said polynucleotide sequence hybridized to a polynucleotide or oligonucleotide probe, said probe having attached thereto a chemical label further comprising a signalling moiety capable of generating a soluble signal;
- and
- generating and detecting said soluble signal.
Dependencies are inferred from the claim text.