Method for protein and peptide sequencing using derivatized glass supports
AssigneePorton Instruments, Inc.
InventorVincent Farnsworth
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Abstract
Peptides or proteins are sequenced by stepwise degradation while immobilized on a glass support derivatized with a silica-binding substance bearing a free acid group, especially a sulfonic acid group. The support is preferably derivatized with 2-(4-chlorosulfonyl phenyl) ethyl trimethoxysilane. Peptide sequencing performance is improved if the support is also derivatized with a monomeric silica-binding substance bearing a free quaternary ammonium group, such as N-trimethyoxysilyl propyl -N,N,N-trimethyl ammonium chloride.
Claims13
In a method of sequencing a peptide or protein which comprises immobilizing the peptide or protein on a support and sequencing the peptide or protein by stepwise degradation, the improvement which comprises providing a glass support derivatized with a silica-binding substance having a free acid group, said acid having a pKa<1, said derivatized support being capable of retaining said peptide or protein.
In a method of sequencing a peptide or protein which comprises immobilizing the peptide or protein on a support and sequencing the peptide or protein by stepwise degradation, the improvement which comprises immobilizing the peptide or protein on a glass support derivatized with a first silica-binding substance having a free sulfonic or halosulfonic acid group, and a second silica-binding substance having a free quaternary ammonium group, wherein said support is capable of retaining a peptide or protein.
In a method of sequencing a peptide or protein which comprises immobilizing the peptide or protein on a support and sequencing the peptide or protein by stepwise degradation, the improvement which comprises providing a glass support derivatized with an organosilane having the formula R.sub.n SiX.sub.(4-n), where n is 1 to 3, X is a hydrolyzable group suitable for covalently bonding the substance to the glass support, and R is a nonhydrolyzable organic radical bearing a quaternary ammonium group, said derivatized support being capable of retaining the peptide or protein.
Dependencies are inferred from the claim text.