Method of producing endo-.alpha.-N-acetylgalactosaminidase from microorganisms
AssigneeSeitetsu Kagaku Co., Ltd.
InventorsTatsurokuro Tochikura, Hidehiko Kumagai, Kenji Yamamoto, Setsu Kadowaki
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Abstract
This invention provides endo-.alpha.-N-acetylgalactosaminidase (endo-.alpha.-GalNAcase) from a microorganism belonging to the genus Alcarigenes. This endo-.alpha.-GalNAcase is very useful in the analysis of the structure and function of mucin-type sugar chains of glycoproteins, as it is an enzyme that cleaves O-glycosidic linkages of sugar chains of glycoproteins, releasing the sugar chain from said protein.
Claims2
A method of producing endo-.alpha.-N-acetyl-galactos-aminidase comprising the steps of growing a microorganism having all of the identifying characteristics of Alcaligenes sp. F-1906 and isolating endo-.alpha.-N-acetylgalactosaminidase from the culture of said microorganism.
Dependencies are inferred from the claim text.