Inhibitors of nonenzymatic cross-linking
AssigneeThe Rockefeller University
InventorsPeter C. Ulrich, Anthony Cerami
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Abstract
The present invention relates to compositions and methods for inhibiting nonenzymatic cross-linking (protein aging). Accordingly, a composition is disclosed which comprises an agent capable of inhibiting the formation of advanced glycosylation endproducts of target proteins by reacting with the carbonyl moiety of the early glycosylation product of such target proteins formed by their initial glycosylation. Suitable agents contain an active nitrogen-containing group, such as a hydrazine group. Particular agents comprise aminoguanidine derivatives. The method comprises contacting the target protein with the composition. Both industrial and therapeutic applications for the invention are envisioned, as food spoilage and animal protein aging can be treated.
Claims15
The compounds of the formula ##STR16## wherein R.sub.1 is hydrogen or a lower alkyl group of 1-6 carbon atoms;
- a hydroxyethyl group;
- n is an integer of 2-7;
- R.sub.3 is hydrogen, a lower alkyl group of 1-6 carbon atoms, R.sub.4 is hydrogen, a lower alkyl group of 1-6 carbon atoms or an amino group;
- R.sub.5 is hydrogen, or a lower alkyl group of 1-6 carbon atoms;
- with the provisio that at least one of R.sub.1, R.sub.3, R.sub.4 or R.sub.5 is other than hydrogen;
- and their pharmaceutically acceptable acid addition salts.
A pharmaceutical composition for administration to an animal to inhibit the advanced glycosylation of a target protein within said animal, comprising a pharmaceutically effective amount of a compound of the formula ##STR17## wherein R.sub.1 is hydrogen or a lower alkyl group of 1-6 carbon. atoms;
- a hydroxyethyl group;
- n is an integer of 2-7;
- R.sub.3 is hydrogen, a lower alkyl group of 1-6 carbon atoms;
- R.sub.4 is hydrogen, a lower alkyl group of 1-6 carbon atoms;
- or an amino group;
- R.sub.5 is hydrogen, or a lower alkyl group of 1-6 carbon atoms;
- with the proviso that at least one of R.sub.1, R.sub.3 R.sub.4 or R.sub.5 is other than hydrogen;
- and their pharmaceutically acceptable acid addition salts together with a pharmaceutically acceptable carrier therefor.
A method for treating an animal to inhibit the formation of advanced glycosylation end products of a target protein within said animal, said method comprising administering an effective amount of a pharamaceutical composition, said pharmaceutical composition comprising a compound of the formula ##STR18## wherein R.sub.1 is hydrogen or a lower alkyl group of 1-6 carbon atoms;
- a hydroxyethyl group;
- n is an integer of 2-7;
- R.sub.3 is hydrogen, a lower alkyl group of 1-6 carbon atoms;
- R.sub.4 is hydrogen, a lower alkyl group of 1-6 carbon atoms or an amino group;
- R.sub.5 is hydrogen, or a lower alkyl group of 1-6 carbon atoms;
- with the proviso that at least one of R.sub.1, R.sub.3, R.sub.4 or R.sub.5 is other than hydrogen;
- and their pharmaceutically acceptable acid addition salts together with a pharmaceutically acceptable carrier therefor.
Dependencies are inferred from the claim text.