Intermediate for and fluorescent cyanine dyes containing carboxylic acid groups
AssigneeCarnegie Mellon University
InventorsPhilip L. Southwick, Alan S. Waggoner
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Abstract
(2,3,3-Trimethyl-3-H-indol-5-yl)-acetic acid has been synthesized. Additionally, derivatives of (2,3,3-Trimethyl-3-H-indol-5-yl)-acetic acid have been prepared as intermediates for preparation of fluorescent cyanine biolabels. A derived dye, 5.5'-Dicarboxymethyl-3,3,3',3'-tetramethyl-1,1'-diethylindocarboc yanine, is a biolabeling substance with a brilliant red fluorescence.
Claims12
The compound having the formula ##STR7##
The compound having the formula ##STR8## where R.sub.1 is --(CH.sub.2).sub.m CH.sub.3, m ranging from 0 to 25, or arylalkyl and X.sup.- is a counterion to N.sup.+.
The compound having the formula ##STR9## where R.sub.3 is --(CH.sub.2).sub.m COOH Y.sup.-, m ranging from 1 to 5, -(CH.sub.2).sub.n SO.sub.3.sup.-, n ranging from 2 to 6 , or -(CH.sub.2).sub.k N.sup.+ (R.sub.4).sub.3 Z, k ranging from 1 to 4 and R.sub.4 is -(CH.sub.2).sub.j CH.sub.3, j ranging from 0 to 25, or H,
- where Y.sup.- and Z.sup.- are appropriate counterions to N.sup.+.
The compound having the formula ##STR10##
The compound having the formula ##STR11##
The compound having the formula ##STR12##
Dependencies are inferred from the claim text.