Automatic cycling reaction apparatus and automatic analyzing apparatus using the same

An automatic analyzer using an enzymatic cycling reaction comprising;

• first means including a turntable for supporting a number of reaction vessels along its periphery each of said vessels containing given amounts of a sample and a cycling mixture including enzymes;
• second means for keeping liquids contained in the reaction vessels at a low temperature at which the enzymatic cycling reaction does not substantially occur bringing simultaneously liquids contained in all the reaction vessels at a first temperature at which an enzymatic cycling reaction proceeds, keeping the liquids at said temperature for a given period, holding all the liquids simultaneously at a second temperature at which the enzymatic cycling reaction is stopped due to a loss of activity of the enzymes, and keeping all the liquids simultaneously at a third temperature lower than the second temperature and at which temperature an indicator reaction proceeds;
• third means for delivering a given amount of an indicator reagent into respective reaction vessels after the enzymatic cycling reaction of the liquids in the reaction vessels has been stopped;
• and
• fourth means for measuring fluorescent light emitted from substances produced by the indicator reaction, said second means comprising a reaction tank in which said turntable is arranged, means for circulating a thermostatic liquid through said reaction tank, and means for controlling the temperature of said thermostatic liquid.