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OwnerGordon C. Forrest, Simon J. Rattle +2inventors
InventorsGordon C. Forrest, Simon J. Rattle, Grenville A. Robinson, Hugh A. O. Hill
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In its broadest aspect, the present invention provides a method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at leasts one electrode, in which method a labelled component of the assay medium is, at least in part, magnetically held in the vicinity of the electrode. The electrochemical assay method may include a separation step, whereby bound label may be separated from free label in the assay medium. Preferably the electrochemical assay method will include the step of determining a perturbation in an electrochemical characteristic of components of the assay medium associated with a ligand complexing reaction. Such an assay method is applicable to, for example, antibodies and antigens and suitable labels include redox centers, enzyme labels in the presence of an electron-transfer mediator and electron-transfer mediator labels in the presence of an electron donor or acceptor.
A method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at least one electrode, in which method a labelled component of the assay medium is, at least in part, magnetically held in the vicinity of the electrode.
A method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at least one working electrode, in which method a sample to be tested is brought in contact with the assay medium, the assay medium containing a labelled component which is, at least in part, magnetically held in the vicinity of the working electrode, an electrochemical characteristic of the components of the assay medium associated with a ligand complexing reaction is measured and whether there is, and optionally the extent to which there is, a perturbation in that electrochemical characteristic is determined.
Dependencies are inferred from the claim text.