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US 4,977,143 AGranted
Antibacterial and antitumor agents LL-E33288EPSILON-I and LL-E33288EPSILON-BR
AssigneeAmerican Cyanamid Company
InventorsWilliam J. McGahren, George A. Ellestad
Life of this patent
Estimating the term…
Abstract
Antibacterial and antitumor agents designated LL-E333288.epsilon.-I and LL-E33288.epsilon.-Br and their production by strains of Micromonospora echinospora ssp. calichensis designated NRRL-15839, NRRL-15975 and NRRL-18149, are disclosed.
Claims10
The compound LL-E33288.epsilon.-I, having:
- (a) a molecular formula:
- C.sub.54 H.sub.74 N.sub.3 O.sub.21 IS.sub.2 ;
- (b) an elemental analysis:
- C, 48.37;
- H, 5.72;
- N, 3.04;
- S, 4.98 and I, 9.28;
- (c) an optical rotation [.alpha]sub.D.sup.25 =-14.+-.1.degree. (C, 1.115%, methanol);
- (d) an optical density:
- 0.23 at 10.mu.g/ml in methanol at 230 nm;
- (e) a proton magnetic resonance spectrum as shown in FIG. I of the drawings;
- (f) a carbon-13 magnetic resonance spectrum:
- as shown in FIG. II of the drawings;
- and
- (g) The structure:
- ##STR3##
13 more elements
A process for producing the antibiotic LL-E33288.epsilon.Br having the structure:
- ##STR4## which comprises aerobically fermenting the organism Micromonospora echinospora ssp calichensis NRRL 15839 or its antibiotic-producing mutants NRRL 15975 or NRRL 18149 in a liquid medium containing assimilable sources of carbon, nitrogen, bromine and inorganic salts, until substantial antibiotic activity is imparted to said medium and then recovering the antibiotic therefrom.
Dependencies are inferred from the claim text.