Process for the production of recognins and their chemoreciprocals

A process for the production of a product, Recognin L, which comprises extracting lymphoma tumor cells grown in culture with a neutral buffer by repeated disruption of the cells to solubilize protein fractions, separating from the resulting extract of solubilized proteins the fraction having a pK range of from about 1 to 4, and isolating therefrom a product having a molecular weight of about 8000 as determined by thin layer gel chromatography, said product, Recognin L, characterized by forming a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at an alkaline pH, having a spectrophotometric absorption peak wave length of 280 mu, a molecular weight of about 8000 and further having an amino acid composition approximately as follows:

• ______________________________________ Approximate Number of Residues ______________________________________ Aspartic Acid 8 Threonine 5 Serine 5 Glutamic Acid 10 Proline 5 Glycine 13 Alanine 10 Valine 6 1/2 Cysteine 1 Methionine 1 Isoleucine 4 Leucine 7 Tryosine 1 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 Approximate Total = 92 ______________________________________
• the amino acids cysteic acid, hydroxyproline, norleucine, ammonia, isodesmosine, desmosine, hydroxylysine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.