Expression of wild type and mutant glutamine synthetase in foreign hosts

A mutant alfalfa glutamine synthetase enzyme which is resistant to inhibition by a herbicidal glutamine synthetase inhibitor wherein said glutamine synthetase enzyme has the following amino acid sequence:

• __________________________________________________________________________ 1 M S L L S D L I N L D L S E T T E K I I 21 A E Y I W I G G S G L D L R S K A R T L 41 P G P V T D P S Q L P K W N Y D G S S T 61 G Q A P G E D S E V I I Y P Q A I F K D 81 P F R R G N N I L V M C D A Y T P A G E 101 P I P T N K R H A A A K I F S H P D V V 121 A E V P W Y G I E Q E Y T L L Q K D I N 141 W P L G W P V G G F P G P Q G P Y Y C G 161 A G A D K A F G R D I V D S H Y K A C L 181 Y A G I N I S G I N G E V M P G Q W E F 201 Q V G P S V X.sup.1 I S A G D E I W V A R Y I 221 L E R I T E V A G V V L S F D P K P I K 241 G D W N X.sup.2 A G A H T N Y S T K S M R E D 261 G G Y E V I L K A I E K L G K K H K E H 281 I A A Y G E G N E R R L T G R H E T A D 301 I N T F L W G V A N R G A S I R V G R D 321 T E K A G K G Y F E D X.sup.3 R P S S N M D P 341 Y V V T S M I A D T T I L W K P __________________________________________________________________________
• where x.sup.1 at 207 is G;
• X.sup.2 at 245 represents an amino acid other than glycine;
• and
• X.sup.3 at 332 is arginine or lysine.

A mutant alfalfa glutamine synthetase enzyme which is resistant to inhibition by a herbicidal glutamine synthetase inhibitor, wherein said enzyme has the formula:

• __________________________________________________________________________ 1 M S L L S D L I N L D L S E T T E K I I 21 A E Y I W I G G S G L D L R S K A R T L 41 P G P V T D P S Q L P K W N Y D G S S T 61 G Q A P G E D S E V I I Y P Q A I F K D 81 P F R R G N N I L V M C D A Y T P A G E 101 P I P T N K R H A A A K I F S H P D V V 121 A E V P W Y G I E Q E Y T L L Q K D I N 141 W P L G W P V G G F P G P Q G P Y Y C G 161 A G A D K A F G R D I V D S H Y K A C L 181 Y A G I N I S G I N G E V M P G Q W E F 201 Q V G P S V X.sup.1 I S A G D E I W V A R Y I 221 L E R I T E V A G V V L S F D P K P I K 241 G D W N X.sup.2 A G A H T N Y S T K S M R E D 261 G G Y E V I L K A I E K L G K K H K E H 281 I A A Y G E G N E R R L T G R H E T A D 301 I N T F L W G V A N R G A S I R V G R D 321 T E K A G K G Y F E D X.sup.3 R P S S N M D P 341 Y V V T S M I A D T T I L W K P __________________________________________________________________________
• where X.sup.1 at 207 is G;
• X.sup.2 at 245 represents an amino acid other than glycine;
• and
• X.sup.3 at 332 is lysine;
• and wherein said enzyme lacks the four native N-terminal amino acid residues 2 to 5, inclusive.

A mutant alfalfa glutamine synthetase enzyme having the following amino acid sequence:

• __________________________________________________________________________ 1 M S L L S D L I N L D L S E T T E K I I 21 A E Y I W I G G S G L D L R S K A R T L 41 P G P V T D P S Q L P K W N Y D G S S T 61 G Q A P G E D S E V I I Y P Q A I F K D 81 P F R R G N N I L V M C D A Y T P A G E 101 P I P T N K R H A A A K I F S H P D V V 121 A E V P W Y G I E Q E Y T L L Q K D I N 141 W P L G W P V G G F P G P Q G P Y Y C G 161 A G A D K A F G R D I V D S H Y K A C L 181 Y A G I N I S G I N G E V M P G Q W E F 201 Q V G P S V X.sup.1 I S A G D E I W V A R Y I 221 L E R I T E V A G V V L S F D P K P I K 241 G D W N X.sup.2 A G A H T N Y S T K S M R E D 261 G G Y E V I L K A I E K L G K K H K E H 281 I A A Y G E G N E R R L T G R H E T A D 301 I N T F L W G V A N R G A S I R V G R D 321 T E K A G K G Y F E D X.sup.3 R P S S N M D P 341 Y V V T S M I A D T T I L W K P __________________________________________________________________________
• where X.sup.1 at 207 is G;
• X.sup.2 at 245 represents an amino acid other than glycine;
• X.sup.3 at 332 is arginine or lysine;
• and
• wherein said amino acid sequence lacks the methionine residue at position 1.