Spectroscope apparatus and reaction apparatus using the same

A method of monitoring a biochemical reaction, comprising the steps of:

• adding a pigment capable of staining a desired tissue in a cell to the cell;
• illuminating the cell with a predetermined light to generate fluorescence from the pigment;
• separating the fluorescence by way of light dispersing means into spectral components;
• mixing that part of the spectral components which exists in a desired wavelength range by way of the light dispersing means;
• and
• forming a mixed light image of the tissue to observe the tissue with the aid of the image.

A method of determining a quantity of a metal ion in a solution, comprising the steps of:

• injecting a solution containing a metal ion into a frame;
• separating light from the frame by way of light dispersing means into spectral components;
• mixing those spectral components which exists in a predetermined wavelength range by way of the light dispersing means;
• forming an image of mixed light;
• and
• measuring light intensity of the image.

A spectroscope apparatus comprising:

• a first slight which allows light under analysis to pass along a first light path;
• a first plane mirror which reflects the light passed through said first slit along the first light path;
• a first concave mirror which reflects the light reflected by said first plane mirror along the first light path;
• a diffraction grating, disposed at a focus point of the light reflected by said first concave mirror, for producing a spectrum corresponding to the light reflected by said first concave mirror;
• a second concave mirror which reflects the spectrum produced by said diffraction grating along the first light path;
• a second plane mirror which reflects the light reflected by said second concave mirror along the first light path;
• a second slight which allows the light reflected from said second plane mirror to pass along the first light path;
• and
• a third plane mirror for one of reflecting the light passed through said second slit along a second light path different from the first light path so that the light reflected by said third plane mirror impinges on photoelectric conversion means for measuring spectral intensity of light incident thereon, and reflecting the light passed through said second slit along a third light path, different from the first and second light paths, the third light path being a path wherein light, in order, passes through said second slit, reflects off of said second plane mirror, reflects off of said second concave mirror, reflects off of said diffraction grating, reflects off of said first concave mirror and impinges on an image detector for detecting the light reflected by said first concave mirror, said diffraction grating mixing the light reflected by said second concave mirror along the third light path to provide the mixed light to said first concave mirror.